Aim. To determine genotype and allele disribution for the IFNL4 gene ss469415590 and examine it for linkage with the IL28B gene rs12979860 in Ukrainian population. Methods. The studied group consisted of 100 unrelated donors of Eastern European origin representing the population of Ukraine. Genotyping for the IFNL4 gene ss469415590 was performed using the amplification-refractory mutation system PCR. Genotyping for the IL28B gene rs12979860 was performed by the PCR-based restriction fragment length polymorphism assay. Results. Genotype frequencies for both studied variants showed no significant deviation from those expected according to Hardy-Weinberg equilibrium. Allelic distribution for ss469415590 was: TT - 0.665, [delta]G - 0.335. Allelic frequencies of rs12979860 were: C - 0.655, T - 0.345. The results of likelihood ratio test indicated a linkage disequilibrium between the studied variants (p > 0.0001), the major alleles ss469415590 TT and rs12979860 C were in phase. The genetic structure of Ukrainian population in terms of two studied polymorphic variants is similar to the European population presented in the "1000 genomes" project. Conclusions. Considering a tight linkage revealed in Ukrainian population between the ss469415590 variant and& rs12979860, a crucial genetic marker of chronic hepatitis C treatment efficiency, this polymorphism might be a promising target for further investigation as a pharmacogenetic marker.
З 31.12.2014 по 01.03.2015 Наукова бібліотека читачів не обслуговує.
