Система кокультивування була розроблена для вивчення взаємодії між клітинами MCF-7 раку молочної залози та MT-4 (лінії Т-кліттинної лейкемії) за допомогою паракрінних механізмів (без контактне культивування), реактивності та чутливості клітин у різних клітинних популяціях.
A co-culture system was developed to investigate the interactions between MCF-7 breast epithelial cells and MT-4 (line human T-cell leukaemia) by paracrine mechanism (no contact coculture), reactivity and sensibility cells in different cell populations.For spheroids generation was chosen breast adenocarcinoma cell line MCF-7. For coculture - line human T-cell leukaemia MT-4 as source of paracrine influence. MCF-7 viability, allocation and proliferation were determinated in two fractions of MCF -7 cells from spheroid culture and monolayer. In results MT-4 reduced number of cells MCF-7 in fraction of adhesive cells and didn"t influence on suspension fraction. Cells viability was different in this two fraction, in monolayer and spheroids, with MT-4 and without MT-4. MT-4 stimulated proliferation in adhesive fraction and supported proliferation in suspension. Proliferation reduced in both fractions without MT-4. We think that, in dependence of cell interaction in population influ&ence of MT-4 by paracrine signal may stimulate proliferation MCF-7 cells, reduce adhesive to the bottom and lead to formation MTS. In the same time MT-4 supported proliferation cells in suspension but didn"t influence on growth and further evolution &spheroids.
