The best results for Beet necrotic yellow vein virus (BNYVV) purification were obtained from Chenopodium quinoa leaf tissue mechanically inoculated with the virus using slightly modified method described by Bouzoubaa [1]. The presence of BNYVV was confirmed by immunosorbent electron microscopy (IEM) and Western blot analysis. IEM with antiserum to BNYVV coat protein (CP) revealed virus particles morphology typical for rhizomania agent [8]. Purified virus suspension was used for CP evaluation and detection by Western blot.
