According to the internationally agreed joint project "Investigation of distribution of plum pox virus in Lithuania and Ukraine and development of biotechnological methods of obtaining of virus-free planting material" 95 samples of plum, apricot and peach from several locations of Ukraine were collected in June 2007 and checked for the presence of PPV by DAS-ELISA. Presence of PPV was detected in all explored regions. Four isolates PPV from plum of Kyivsky and Transkarpathian regions were transferred to herbaceous plants and the presence of the virus in these plants was confirmed by DAS-ELISA and IEM investigation. The presence of PPV in samples from Lithuania was confirmed in 2 cases from 20. PPV positive samples were collected from Jonava and Sakiairegions.