Screening of Cactaceae plants on virus diseases in the collections of Fomin"s Botanical garden of Taras Shevchenko Kyiv National University and Botanical garden of Karasin"s Kharkiv National University have been conducted. Opuntia plants were the most infected in both collections. Basing on serological, biological and morphological properties, we suggest that isolated virus is related to Cactus virus 2.