Distribution of Apple mosaic virus, Apple chlorotic leaf spot virus, Apple stem pitting and Apple stem growing virus have been investigated in fruit orchards of Kiev region and the City of Kiev in 2007. The widest spread was shown for Apple chlorotic leaf spot virus (68.4%). Three different techniques for extraction of total RNA preparations from apple plant material for subsequent RT-PCR were compared. Method proposed by Kundu (2001) has been shown to be most efficient according to the quantity of obtained RNA preparation.